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In Vitro Cancer Biology Services

SRI offers our clients several in vitro assays to help determine the efficacy, mechanism of action, and toxicity of their compounds. We have numerous human cell lines derived from such cancers as lung, stomach, colon, breast, prostate, ovary, liver, skin, leukemia, and lymphoma. Whenever possible, we obtain cell lines from our clients to decrease nonreproducible results due to variants arising from multiple passages of cells. If the client desires a specific cell line not in our library, we will determine its availability from sources such as the American Type Culture Collection (ATCC).

We offer in vitro

• Cell proliferation and growth inhibition assays (tumor and endothelial cells)
• Apoptosis assays (tumor and endothelial cells)
• Cell cycle and apoptosis analysis using flow cytometry
• Migration and invasion assays (Matrigel® and fibrin gel)
• Receptor binding and activity assays (ER, PR, AR, PPAR, etc.)
• ELISA, Western blots on treated animal tissue and cell lysates for enzymes, cytokines and other protein targets
• Zymogels for protease activity (MMPs, etc.)
• RNA isolation, PCR, RT-PCR and SuperArray®
• Biochemical assays for target effectiveness on treated animal tissues
• Custom in vitro assays

Many times clients have a number of compounds of interest and need in vitro efficacy data to select the best compounds to move forward into animal studies. In addition to efficacy assays, SRI offers toxicity assays to estimate the absorption, metabolism, and toxicity of the compounds of interest. The results from in vitro assessment can be used to select the best lead candidates and design the appropriate in vivo efficacy experiments.

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       Last Updated Mar 19, 2009